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Your Position: Home > Antibody > CD16a > CDA-PFM785

PE-Labeled Monoclonal Anti-CD16a Antibody, Mouse IgG1 (3H8B10) (0.03% Proclin)

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  • Source
    Monoclonal Anti-CD16a Antibody, Mouse IgG1 (3H8B10) is a mouse monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.
  • Application
    Flow Cytometry (Evaluation of the expression of CD16a on Human cells).
  • Clone
    3H8B10
  • Species
    Mouse
  • Isotype
    Mouse IgG1 | Mouse Kappa
  • Specificity
    This product is a specific antibody specifically reacts with CD16a protein. ACRObiosystems offers two anti-CD16 antibodies (clones 3H8B10 and 3G8) that target distinct epitopes on CD16.
  • Reactivity

    Human

  • Immunogen

    Purified Human CD16a Protein

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Isotype Control

    The Isotype control is sold separately and you can search for Cat. No. DNP-PM1 for product information.

  • Recommended Dilution
    1:20
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin300 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
Bioactivity-FACS
 CD16a FACS

Flow cytometric analysis of human peripheral blood lymphocytes respectively staining with PE-Labeled Monoclonal Anti-CD16a Antibody, Mouse IgG1 (3H8B10) (Cat. No. CDA-PFM785) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) and FITC anti-human CD56 Antibody, compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).

 CD16a FACS

Flow cytometric analysis of Human peripheral blood mononuclear cell respectively staining with PE-Labeled Monoclonal Anti-CD16a Antibody, Mouse IgG1 (3H8B10) (Cat. No. CDA-PFM785) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).

  • Background
    CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
  • Clinical and Translational Updates

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