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| Assay Type | Sandwich-ELISA |
| Analyte | Fibronectin |
| Format | 96T (8×12 strips) |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | ＜100 pg/mL |
| Standard Curve Range | 100 pg/mL-3200 pg/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the detection and quantitative determination of Human Fibronectin in cell culture supernates. |
| Sample volume | 100 uL |
To support the development of cell therapy drugs, ACROBiosystems developed Human fibronectin quantitive ELISA Kit with rigorous methodological validation, which is used for the detection and quantitative determination of fibronectin in cell culture supernates, serum, and plasma. Besides, this kit can also be used for the quantitative determination of Recombinant Fibronectin fragment, premium grade (ACROBiosystems, cat# GMP-FINH18) concentrations.
The kit is developed for the detection and quantitative determination of Fibronectin in cell culture supernates.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| RES108-C01 | Pre-coated Anti-Human Fibronectin Antibody Microplate | 1 plate(8×12 strips) |
| RES108-C02 | Human Fibronectin Standard | 5 μg×2 |
| RES108-C03 | Biotin-Anti-Human Fibronectin Antibody | 20 μg |
| RES108-C04 | Streptavidin-HRP | 50 μL |
| RES108-C05 | 10×Washing Buffer | 50 mL |
| RES108-C06 | 2×Dilution Buffer | 50 mL |
| RES108-C07 | Substrate Solution | 12 mL |
| RES108-C08 | Stop Solution | 7 mL |
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
To assess the linearity of the assay, samples spiked with high concentrations of human Fibronectin were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Five parts of blank T cell culture supernatant were added with different concentrations of human Fibronectin, and the T cell culture supernatant without human Fibronectin was used as background to calculate the recovery rate. The range of the recovery rate is 82.5-93.7%, and the average recovery is 89.3%.
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