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Your Position: Home > Kits > Spike protein > RAS-T023

Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)

For research use only.

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    Materials Provided
    IDComponentsSize
    RAS023-C01Pre-coated SARS-CoV-2 Spike Protein Microplate1 plate
    RAS023-C02Anti-SARS-CoV-2 Antibody (Control, Mouse IgG )100 μL
    RAS023-C03HRP-Conjugated Antibody10 μg
    RAS023-C0410 x Washing Buffer 50 mL
    RAS023-C05Dilution Buffer50 mL
    RAS023-C06Substrate Solution12 mL
    RAS023-C07Stop Solution7 mL
    RAS023-C08SARS-CoV-2 Antibody Positive Control100 μL
    RAS023-C09SARS-CoV-2 Antibody Negative Control100 μL
  • Application

    This kit is developed for detection of Mouse Anti-SARS-CoV-2 antibody (IgG) in serum in vitro.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This kit is based on a standard indirect ELISA assay that allows rapid detection of Anti-SARS-CoV-2 antibodies (IgG) in mouse serum by SARS-CoV-2 Spike Protein. The kit consists of a Microplate Pre-coated with SARS-CoV-2 Spike Protein, Anti-SARS-CoV-2 Antibody (Control, Mouse IgG), and HRP-Goat-Anti-Mouse IgG Secondary Antibody.

    Your experiment will include 5 simple steps:

    a) Add your diluted Samples and Control to the plate.

    b) Add diluted Secondary Antibody HRP-Goat-Anti-Mouse IgG to the plate.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by adding diluted acid.

    e) To remove background noise prior to statistical analysis, absorbance (OD) is calculated by substracting the value at 450 nm with the value at 630 nm. The OD Value reflects the amount of antibody bound.

Typical Data Please refer to DS document for the assay protocol.
 Spike protein TYPICAL DATA

Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Protein at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG in 1:100 mouse serum. Detection was performed using HRP-Anti-mouse IgG antibody with sensitivity of 20 ng/mL (QC tested).

  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $580.00

Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Approved

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