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| Product | Size | Amount |
| ActiveMax® Human BCMA μBeads, premium grade (for cells) | 2.5 mg | 2.5×10⁷ beads |
| ActiveMax® Human BCMA μBeads, premium grade (for cells) | 10 mg (2.5 mg×4) | 1.0×10⁸ beads |
See Certificate of Analysis (CoA) for detailed instruction.
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Assay of human BCMA protein on the μBeads surface by Flow cytomtry. The human BCMA conjugated on the μBeads (Cat. No. MBS-C004) surface were fluorescently stained using PE labeled anti-human BCMA antibody and analyzed by flow cytometry.
Add 100ul of 1:40 PE anti-human BCMA Antibody dilution (2.5ul stock solution in 100ul FACS buffer) into 5e5 of ActiveMax® human BCMA μBeads, premium grade (for cells) (Cat. No. MBS-C004), negative control beads (ActiveMax® Streptavidin μBeads, premium grade (for cells) (Cat. No. MBS-C009)) as well. PE signals was used to evaluate BCMA beads binding activity.
Add 100ul of 1:40 PE anti-human BCMA Antibody dilution (5ul stock solution in 100ul FACS buffer) into 5e5 of ActiveMax® human BCMA μBeads, premium grade (for cells) (Cat. No. MBS-C004), Negative control (ActiveMax® Streptavidin μBeads, premium grade (for cells) (Cat. No. MBS-C009)) as well. PE signals was used to evaluate BCMA beads binding activity.
ActiveMax® Human BCMA μBeads, premium grade (for cells) (Cat.No.MBS-C004) can activate BCMA-specific CAR-T cells by detecting the secretion of IFN- γ in vitro.
BCMA CAR-T cells were stimulated with ActiveMax® Human BCMA μBeads (MBS-C004) for 24 h , and cell-free supernatants were harvested for evaluating IFN-γ、TNF-α、IL-4、IL-6 secretion by flow cytomtry. The results showed that BCMA CAR-T cells released significantly larger amounts of IFN-γ、TNF-α、IL-4、IL-6 into the supernatants in response to BCMA μBeads.
BCMA CAR-T cells were stimulated with ActiveMax® Human BCMA μBeads (MBS-C004) for 24h, and cell-free supernatants were harvested for evaluating Granzyme B, Perforin secretion by ELISA. The results showed that BCMA CAR-T cells released significantly larger amounts of Granzyme B, Perforin into the supernatants in response to BCMA μBeads.
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ACROBiosystems developed a series of GMP grade cytokines under the GMP grade quality management system. Those products are all suitable for T/NK cell generation, activation, and proliferation in cell therapy research.
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GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
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To support preclinical/clinical immunogenicity and PK analysis, ACROBiosystems has developed a series of high-affinity anti-idiotypic antibodies. Our pipeline covers five hot targets including adalimum*b, rituxim*b, cetuxim*b, trastuzum*b, and bevacizum*b.
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