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| Assay Type | Sandwich-ELISA |
| Analyte | Protein A |
| Format | 96T(8×12 strips) |
| Regulatory Status | RUO |
| Sensitivity | ＜40pg/mL |
| Standard Curve Range | 50 pg/mL-1600 pg/mL(Protein A) 50 pg/mL-3200 pg/mL(PrismA) |
| Assay Time | 2 hr |
| Suitable Sample Type | For the quantitative determination of recombinant Protein A, alkaline-resistant Protein A, PrismA and MaXtar® ARPA ligand Protein A. |
| Sample volume | 25 μL |
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| RES029-C01 | Pre-Coated Anti-Protein A Antibody Microplate | 1 plate(8×12 strips) |
| RES029-C02A | Alkali-Tolerant Recombinant Protein A Standard (1μg/mL) | 100 μL |
| RES029-C02B | MaXtar® ARPA ligand Protein A Standard (Bio-Link Co.) (1μg/mL) | 100 μL |
| RES029-C02C | Recombinant PrismA Standard (1μg/mL) | 100 μL |
| RES029-C03 | Recombinant Protein A Standard (1μg/mL) | 100 μL |
| RES029-C04 | Biotin-Anti-Protein A Antibody | 1.5 mL |
| RES029-C05 | Streptavidin-HRP | 10 μg |
| RES029-C06 | 10×Sample Dilution Buffer | 15 mL |
| RES029-C07 | Denaturation Buffer | 15 mL |
| RES029-C08 | 20×Washing Buffer | 30 mL |
| RES029-C09 | Antibody Dilution Buffer | 15 mL |
| RES029-C10 | Streptavidin-HRP Dilution Buffer | 15 mL |
| RES029-C11 | Substrate Solution | 12 mL |
| RES029-C12 | Stop Solution | 8 mL |
Detection of Recombinant Protein A by sandwich-ELISA Assay.
Immobilized Anti-Protein A Antibody can bind Recombinant Protein A. Detection was performed using Biotin-Anti-Protein A Antibody with sensitivity of 50 pg/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Detection of Alkali-Tolerant Recombinant Protein A by sandwich-ELISA Assay.
Immobilized Anti-Protein A Antibody can bind Alkali-Tolerant Recombinant Protein A. Detection was performed using Biotin-Anti-Protein A Antibody with sensitivity of 50 pg/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Detection of MaXtar® ARPA ligand Protein A (Bio-Link Co.) by sandwich-ELISA Assay.
Immobilized Anti-Protein A Antibody can bind MaXtar® ARPA ligand Protein A (Bio-Link Co.). Detection was performed using Biotin-Anti-Protein A Antibody with sensitivity of 50 pg/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Detection of Recombinant PrismA by sandwich-ELISA Assay.
Immobilized Anti-Protein A Antibody can bind Recombinant PrismA. Detection was performed using Biotin-Anti-Protein A Antibody with sensitivity of 50 pg/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Four samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Four samples of known concentration were tested in three separate assays to assess inter-assay precision.
Add different concentrations of Protein A (0.2ng/mL、1ng/mL、10ng/mL) to different concentrations of Human IgG1 (Bevacizumab) (20mg/mL、10mg/mL、5mg/mL) or Human IgG4 (Toripalimab) (20mg/mL、10mg/mL、5mg/mL), then dilute the antibodies to a reasonable range, then test and calculated the concentration of protein A to give the recovery rate.
Add Recombinant Protein A to Human IgG1 (Bevacizumab) or Human IgG4 (Toripalimab):
Add Alkali-Tolerant Recombinant Protein A to Human IgG1 (Bevacizumab) or Human IgG4 (Toripalimab):
Add MaXtar® ARPA ligand Protein A (Bio-Link Co.) to Human IgG1 (Bevacizumab) or Human IgG4 (Toripalimab):
Add Recombinant PrismA to Human IgG1 (Bevacizumab) or Human IgG4 (Toripalimab):
We have conducted interference effect test about frequently-used buffers, they have excellent buffer compatibility. For specific buffers, it is recommended that you verify recovery to determine the minimum dilution ratio.
Host cell protein (HCP 500 ng/mL) and host cell DNA (HCD 0.5 ng/mL) of HEK293, E.coli or CHO systems were added to human IgG1 (Bevacizumab, 1mg/mL) and human IgG4 (Toripalimab, 1mg/mL), respectively, which were higher than the usual quality standard limit. Then high, medium, and low concentrations of Protein A were added, respectively, and the ratio of Protein A recovery in the Protein A added samples without HCP and HCD was added as the specificity verification index. verification index.
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