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5e5 of Jurkat cells were stained with 100 μL of 0.3 μg/mL of Biotinylated Anti-Human CD3 Antibody, Mouse IgG2a,Avitag (Clone: OKT3), premium grade (Cat. No. CDE-BV84G0) and isotype control antibody respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
2e5 of Jurkat cells were stained with 100 μL of 1 μg/mL of Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1) (Cat. No. CDE-M532) and isotype control antibody respectively, washed and then followed by PE-anti mouse IgG1 antibody and analyzed with FACS (Routinely tested).
Immobilized Human CD3E&CD3D Heterodimer Protein (Cat. No. CDD-H52Wa) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (UCHT1) (Cat. No. CDE-M532) with a linear range of 0.1-3 ng/mL (QC tested).
The purity of Biotinylated Anti-Human CD3 Antibody, Mouse IgG2a,Avitag (Clone: OKT3), premium grade (Cat. No. CDE-BV84G0) is more than 95% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
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