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Your Position: Home > Antibody > CD3 > CDE-CH64G0

Monoclonal Anti-Human CD3 Antibody, Human IgG4 (Clone: OKT3), premium grade

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  • Source
    Monoclonal Anti-Human CD3 Antibody, Human IgG4 (Clone: OKT3), premium grade (CDE-CH64G0) is recombinantly produced from CHO cells.
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Isotype
    Human IgG4 | Human Kappa
  • Conjugate

    Unconjugated

  • Specificity
    This product is a specific antibody specifically reacts with CD3 epsilon.
  • Endotoxin
    Less than 0.002 EU per μg by the LAL method / rFC method.
  • Protein A
    <5 ppm of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Sterility
    Negative
  • Mycoplasma
    Negative
  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    For long term storage, the product should be stored at liquid state at -70°C.

    This product is stable after storage at:

    1. 2-8°C for 12 months under sterile conditions;
    2. -70°C for 24 months.
SDS-PAGE
CD3 SDS-PAGE

Monoclonal Anti-Human CD3 Antibody, Human IgG4 (Clone: OKT3), premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD3 MALS images

The purity of Monoclonal Anti-Human CD3 Antibody, Human IgG4 (Clone: OKT3), premium grade (Cat. No. CDE-CH64G0) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

Bioactivity-CELL BASE
 CD3 CELL

Monoclonal Anti-Human CD3 Antibody, Human IgG4 (Clone: OKT3), premium grade (Cat. No. CDE-CH64G0) stimulates secretion of IL-2 by PBMC stimulated with Monoclonal Anti-Human CD28 Antibody, Mouse IgG1. The typical EC50 for this effect is 0.1442 ng/mL (QC tested).

Bioactivity-ELISA
 CD3 ELISA

Immobilized Human CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-H52W5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Human IgG4 (Clone: OKT3), premium grade (Cat. No. CDE-CH64G0) with a linear range of 0.06-4 ng/mL (QC tested).

  • Background
    CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.
    Immunoglobulin 4 (IgG4) is one of four human IgG subclasses and has several unique functional characteristics. It exhibits low affinity for complement and for most Fc receptors. It furthermore has generally high affinity for its antigen, with binding occurring in a monovalent fashion, as IgG4 can exchange Fab-arms with other IgG4 molecules. Because of these characteristics, IgG4 is believed to block its targets and prevent inflammation, which, depending on the setting, can have a protective or pathogenic effect.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:7 Details
  • Number of Drugs in Clinical Trials:159 Details
  • Latest Research Phase:Approved

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