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Your Position: Home > Kits > AAV9 > AAV-A009

AAV9 Titration ELISA Kit

For research use only.

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    Materials Provided
    IDComponentsSize
    AAV09-C01Pre-coated Anti-AAV9 Antibody Microplate1 plate
    AAV09-C02AAV9 Standard3.80E+11 capsids
    AAV09-C03Biotin-Anti-AAV9 Antibody20 μg
    AAV09-C04Streptavidin-HRP50 μL
    AAV09-C0510×Washing Buffer 50 mL
    AAV09-C062×Dilution Buffer50 mL
    AAV09-C07Substrate Solution12 mL
    AAV09-C08Stop Solution7 mL
  • Product Overview
    AAV9 Titration ELISA Kit is based on the sandwich-ELISA method and is used to detect and quantitatively determine the AAV9 capsid titration in AAV gene therapy product. The kit is designed to provide reliable solutions for the early development, production, and quality control of AAV gene therapy products.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Typical Data Please refer to DS document for the assay protocol.
     AAV9 TYPICAL DATA

    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

    Validation
    Dilution Linearity

    To assess the linearity of the assay, samples spiked with high concentrations of AAV9 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

     AAV9 DILUTION LINEARITY
    Intra-Assay Statistics

    Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.

     AAV9 INTRA-ASSAY STATISTICS
    Inter-Assay Statistics

    Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

     AAV9 INTER-ASSAY STATISTICS
    Recovery

    Three AAV9 with different concentrations were tested to calculate the recovery rate.

     AAV9 RECOVERY
    Specificity

    The specific binding of AAV-A009 to AAV1/2/3/5/6/8/9/dj serotypes was detected. The results showed that AAV-A009 only binds specifically to AAV9.

     AAV9 SPECIFICITY

    The specific binding of AAV-A009 to native/denatured AAV9 was detected. The results showed that AAV-A009 only binds specifically to native AAV9. (Denatured AAV9: 95℃ for 5min)

     AAV9 SPECIFICITY
    • Background: AAV9
      The adeno-associated virus (AAV) is a small (25 nm), non-enveloped virus of the parvoviridae family, including 12 different AAV serotypes. In the parvoviridae family it belongs to the genus dependoparvovirus, because it needs the presence of a helper virus for replication and assembly. The icosahedral AAV capsid composed of the capsid proteins VP1, VP2 and VP3 contains a linear, single-stranded DNA genome of 4.7 kb.
    • Clinical and Translational Updates

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