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Use this kit to detect residual DNA after the DNA is extracted from test samples. For achieving the better DNA recovery, it is recommended to use the resDetect™ resDNA Sample Preparation Kit (Magnetic Beads) (Cat. No. OPA-R005) in combination.
The kit is used for quantitative detection of residual CHO DNA in biopharmaceutical production.
For use in quality control/manufacturing process only.
It is for research use only.
High sensitive: LLOQ (Lower limit of quantitation) is 3 fg/μL
Figure 1. High sensitivity and broad dynamic range using the CHO resDNA Quantitation Kit. (A) Typical analysis results obtained with Standard 1 (300 pg/μL) to 6 (3 fg/μL). (B) The standard curve of the 10-fold dilution series. PCR efficiency should be 90-110%.
High specificity: No cross-reactivity with unrelated DNA
Figure 2. Assay specificity. Standard curves generated using 10-fold serial dilution of CHO genomic DNA (included in the kit).
High consistency：Consistent performance across the expected range of DNA fragment sizes
Figure 3. Consistent quantitation across a broad range of fragment sizes. Standard curves were generated using a 10-fold serial dilution of gDNA and fragmented DNA. Fragmented DNA was generated by sonicating total CHO genomic DNA (10 min, 20 min, 30min).
The validation of standard curve
Figure 4. The amplification efficiency of standard curve (ACRO and Competitor J) is close to 100%, while the amplification efficiency of standard curve (Competitor H) is less than 90%.
The validation of spike recovery
Figure 5. The validation of spike recovery in three spike levels CHO DNA samples (high, medium, low). Results show that the recovery using the ACRO products are between 90 and 100%.
Complex Sample Simulation Testing
For samples with high concentration BSA (up to 150 mg/mL) and different pH buffers (3.0 ≤ pH ≤ 10.0), the resDetectTM kits were used for the extraction and detection of CHO resDNA. The acceptable spiked recovery range should be 50-150%. The results showed that the spiked recoveries were all between 98-120%. The results are shown in the table below.
Testing of complex samples from CHO antibody purification process
For samples from different steps (AF, VI, AEX, and DF) in the CHO antibody purification process development , the extraction and detection of CHO resDNA were performed using the resDetectTM kits. The acceptable spiked recovery range is 50-150%. The results showed that the spiked recoveries for the various intermediate samples were all close to 100%.
| ID | Components | Size |
| OPA-R004-01 | 2×qPCR Master Mix | 1.0 mL×2 |
| OPA-R004-02 | CHO Primer & Probe Mix | 700 μL |
| OPA-R004-03 | CHO DNA Control（3ng/μL） | 100 μL |
| OPA-R004-04 | Dilution Buffer | 1.5 mL×3 |
| OPA-R004-05 | DNase/RNase-Free Water | 1.0 mL |
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