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Your Position: Home > Kits > AAV8 > PAV-A008

Anti-AAV8 Antibody ELISA Kit

For research use only.

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    Product Specifications
    Assay TypeSandwich-ELISA
    AnalyteAAV8
    Format96T
    Reactivity/
    Regulatory StatusRUO
    Sensitivity≤100ng/mL
    Standard Curve Range0.938 ng/mL-60 ng/mL
    Assay Time3 hr 20 min
    Suitable Sample TypeFor the quantitative determination of anti-AAV8 antibodies in serum.
    Sample volume100 uL
  • Product Overview
    Anti-AAV8 Antibody ELISA Kit is based on the sandwich-ELISA method and is used to detect and quantitatively determine the Anti-AAV8 Antibody in serum. The kit is designed to provide reliable solutions for the immunogenicity studies and enrollment screening of AAV gene therapy products.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Materials Provided
    IDComponentsSize
    PAV008-C01Pre-coated AAV8 Capsid Protein Microplate1 plate
    PAV008-C02Anti-AAV8 Antibody Standard2.5 μg
    PAV008-C03Biotin-AAV8 Capsid Protein1 μg
    PAV008-C04Streptavidin-HRP10 μg
    PAV008-C0510xWashing Buffer 50 mL
    PAV008-C062xDilution Buffer50 mL
    PAV008-C07Substrate Solution12 mL
    PAV008-C08Stop Solution7 mL
Typical Data Please refer to DS document for the assay protocol.
 AAV8 TYPICAL DATA

Detection of anti-AAV8 antibodies by sandwich-ELISA Assay.
Immobilized AAV8 Capsid Protein can bind Anti-AAV8 Antibody in 1:50 human serum, and then add Biotin-AAV8 Capsid Protein. Detection was performed using Streptavidin-HRP with sensitivity of 46.9 ng/mL.

 AAV8 TYPICAL DATA

The specific binding of PAV-A008 to Anti-AAV3/5/9 antibody was detected. The results showed that PAV-A008 only binds specifically to Anti-AAV8 antibody.

Validation
Intra-Assay Statistics

Three samples of known concentration in blank human serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.

 AAV8 INTRA-ASSAY STATISTICS

Three samples of known concentration in blank mouse serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.

 AAV8 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Three samples of known concentration in blank human serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.

 AAV8 INTER-ASSAY STATISTICS

Three samples of known concentration in blank mouse serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.

 AAV8 INTER-ASSAY STATISTICS
  • Background: AAV8
    Adeno-associated viruses (AAVs) are being developed as gene therapy vectors, and their efficacy could be improved by a detailed understanding of their viral capsid structures. AAV serotype 8 (AAV8) shows a significantly greater liver transduction efficiency than those of other serotypes, which has resulted in efforts to develop this virus as a gene therapy vector for hemophilia A and familial hypercholesterolemia.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(USD) : $1225.00

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