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Your Position: Home > Protein > Hemagglutinin/HA (Influenza Virus) > H32-V82E3

Biotinylated Influenza A Virus (A/Thailand/8/2022) & (A/Massachusetts/18/2022) HA (H3N2) Protein, His,Avitag™ (MALS verified)

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  • Synonym
    Hemagglutinin/HA (Influenza Virus)
  • Source
    Biotinylated Influenza A Virus HA (H3N2) Protein, His,Avitag (H32-V82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 17 - Asp 529 (Accession # EPI2178977 & EPI2096148, GISAID).
    Predicted N-terminus: Gln 17
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 64.4 kDa. The protein migrates as 95-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a trimer.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Hemagglutinin/HA (Influenza Virus) SDS-PAGE

Biotinylated Influenza A Virus HA (H3N2) Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Hemagglutinin/HA (Influenza Virus) MALS images

The purity of Biotinylated Influenza A Virus HA (H3N2) Protein, His,Avitag (Cat. No. H32-V82E3) is more than 85% and the molecular weight of this protein is around 250-280 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Hemagglutinin/HA (Influenza Virus) ELISA

Immobilized Human Anti-HA (H3N2) antibody CR8020 Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Influenza A Virus HA (H3N2) Protein, His,Avitag (Cat. No. H32-V82E3) with a linear range of 0.2-16 ng/mL (QC tested).

 Hemagglutinin/HA (Influenza Virus) ELISA

Immobilized Biotinylated Influenza A Virus HA (H3N2) Protein, His,Avitag (Cat. No. H32-V82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Anti-HA (H3N2) antibody CR8020 Human IgG1 with a linear range of 0.2-16 ng/mL (Routinely tested).

  • Background
    Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.
  • Clinical and Translational Updates

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