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5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 3 μg/mL of Alexa Fluor 647-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-AM534) and negative control respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa 647 signal was used to evaluate the binding activity (QC tested).
Human CD19 (20-291), Fc Tag (Cat. No. CD9-H5251) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) with an affinity constant of 0.668 nM as determined in a SPR assay (Biacore 8K) (QC tested).
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