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Expression analysis of human uPAR on CHO/Human uPAR Stable Cell Line by FACS.
Cell surface staining was performed on CHO/Human uPAR Stable Cell Line or negative control cell using PE-labeled anti-human uPAR antibody.
5e5 of anti-uPAR CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of APC-Labeled Human uPAR Protein, His Tag (Cat. No. UPR-HA2H9) and negative control protein respectively. APC signal was used to evaluate the binding activity (QC tested).
Mouse uPAR, His Tag (Cat. No. UPR-M52H3) immobilized on CM5 Chip can bind Human PLAU, His Tag (Cat. No. PLU-H5229) with an affinity constant of 14.1 nM as determined in a SPR assay (Biacore 8K) (QC tested).
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