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This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 25.5 kDa. The protein migrates as 40-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CD16a (F176), Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) is more than 90% and the molecular weight of this protein is around 35-54 kDa verified by SEC-MALS.
Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 1.72 μM as determined in a SPR assay (Biacore T200) (QC tested).
Immobilized Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) on SA Chip can bind Rituximab with an affinity constant of 1.33 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Biotinylated Human CD16a (F176), Avitag,His Tag (Cat. No. CDA-H82E8) on SA Biosensor, can bind Rituximab with an affinity constant of 1.29 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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