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Your Position: Home > Kits > Her2 > EHM-V1

ELISA Assay Kit for Anti-HER-2 h-mAb in Mouse Serum

For research use only.

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  • Application

    This kit is developed for detect the anti-HER-2 h-mAb binding to Herceptin (Trastuzumab) for research pharmacokinetics.

    It is for research use only.

  • Reconstitution
    See Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at -25~-15℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    The enclosed ELISA assay kit for anti-HER-2 h-mAb in mouse serum is based on an enzyme immunoassay (ELISA) between recombinant HER-2 protein (ECD) and a biotinylated anti-HER-2 monoclonal antibody. The method employs the principle of competitive ELISA, allowing quantification of monoclonal anti-HER-2 antibodies in mouse serum.

    The assay only involves the following steps:

    1. Coat the plate with human HER-2;

    2. Add the mixture of your sample and biotinylated anti-HER-2 antibody;

    3. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate;

    4. Record the OD readings and analyze the serum concentration.

  • Clinical and Translational Updates

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Price(USD) : $1133.00

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