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Generally usable: Generally usable for removing all forms of DNA and RNA from biological products, reducing viscosity and preventing cell clumping.
Efficient: Efficient reaction, rapid degradation, reduce processing time.
Native: Native structure, tag free, the enzyme activity is more guaranteed.
Ideal: Ideal for DNA and RNA clearance
Ultra pure: Ultra pure: High purity and activity
Specific: Specific nuclease, no protease activity
1. Reagents and solutions preparation
50 mM Tris-HCl, 1 mM MgCl2, pH 8.0 ( * In the case of extensive dilution before use, carrier protein such as 0.1 mg/ml HSA or BSA is generally recommended to avoid any enzyme loss from surface adsorption)
1 mg/ml salmon sperm DNA is dissolved overnight at 4 ℃, in reaction buffer, and is then sonicated on ice to obtain a homogenous solution.
Different dilution of nuclease with reaction buffer.
Trichloroacetic acid (TCA)
2. Standard curve establishment
400 μl substrate + 100 μl enzyme of known activity = 500 μl mixture
Incubate the mixture at 37℃ for 30 min.
Stop the reaction by addition of 400 μl cold TCA and incubate on ice for 10 min.
Centrifuge at 8500g for 5 min.
Measure the absorbance of supernatant at 260 nm.
Lot a standard curve with nuclease of known activities for each set of measurements.
3. Measurement of activity
The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of GENIUS™Nuclease is >1.0 x 10e6 unit/mg protein.
Standard curve of nuclease activity for GENIUS™Nuclease.
Notice: We updated the brand name from Benz™Nuclease to GENIUS™Nuclease. The products are the same and the only change is the brand name in the product name and the label.
The purity of GENIUS™Nucleasee was determined by SDS-PAGE reduced and staining overnight with Coomassie Blue.
The purity of GENIUS™Nuclease (Cat. No. BEE-N3116) is more than 95% as determined by SEC-HPLC.
The result of GENIUS™Nuclease activity.
Price(USD) : $88.00
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Emerging VoCs, Omicron, Delta, Beta, Alpha mutants and so on, including RBD, S trimer, S1, NTD, NP, etc. These mutants are of high purity and bioactivity and can be used to evaluate the efficacy of the antibodies and vaccination.
ACROBiosystems developed a series of GMP grade cytokines under the GMP grade quality management system. Those products are all suitable for T/NK cell generation, activation, and proliferation in cell therapy research.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
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A series of immune checkpoints including classic co-inhibitory and co-stimulatory receptors. The comprehensive catalog contains 100+ targets with various species and tags, and the high-quality proteins are in good batch-to-batch consistency.
To meet the needs of ADCs development, ACROBiosystems can provide: A variety of high-quality target proteins; MMPs/Cathepsin/uPA for cleavable linker; Anti-payload antibodies & anti-idiotypic antibodies for immunogenicity and PK analysis; SPR/BLI analytical and ADA development service.
Comprehensive collection of Fc receptor proteins, including their common variants, which can help expedite your antibody development.
The MABSOL biotinylated protein collection includes more than a hundred commonly studied drug targets and biomarker proteins.
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To support preclinical/clinical immunogenicity and PK analysis, ACROBiosystems has developed a series of high-affinity anti-idiotypic antibodies. Our pipeline covers five hot targets including adalimum*b, rituxim*b, cetuxim*b, trastuzum*b, and bevacizum*b.
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