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Your Position: Home > Protein > IgG2b Fc > IGB-L5204

Llama IgG2b Fc Protein, Tag Free (MALS verified)

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  • Synonym
    IgG2B,IgG2b Fc
  • Source
    Llama IgG2b Fc, Tag Free(IGB-L5204) is expressed from human 293 cells (HEK293). It contains AA Glu 1 - Ser 243 (Accession # AAX73259.1).
    Predicted N-terminus: Glu 1
  • Molecular Characterization
    IgG2b Fc Structure

    This protein carries no "tag".

    The protein has a calculated MW of 27.3 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IgG2b Fc SDS-PAGE

Llama IgG2b Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
IgG2b Fc MALS images

The purity of Llama IgG2b Fc, Tag Free (Cat. No. IGB-L5204) is more than 95% and the molecular weight of this protein is around 53-65 kDa verified by SEC-MALS.

  • Background
    Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis. Ig gamma-2 chain Fc region contains two constant regions of IgG2 H chain (CH2, CH3).
  • Clinical and Translational Updates

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