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This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 20.1 kDa. The protein migrates as 20-22 kDa under reducing (R) condition (SDS-PAGE).
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human FGF basic, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) was greater than 85% as determined by SEC-HPLC.
Immobilized Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 0.6-10 ng/mL (QC tested).
Immobilized Human FGFR2 (IIIb), His Tag (Cat. No. FGB-H5223) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Immobilized Human FGFR2 (IIIc), His Tag (Cat. No. FGC-H5225) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 5-39 ng/mL (Routinely tested).
Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) immobilized on SA Chip can bind Human FGF R1, His Tag (Cat. No. FG1-H5223) with an affinity constant of 96.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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