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Your Position: Home > Protein > Galectin-1 > GA1-H4113

Human Galectin-1 / LGALS1 Protein, Tag Free (MALS verified)

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  • Synonym
    LGALS1,GAL1,GBP,Galectin-1,DKFZp686E23103
  • Source
    Human Galectin-1, Tag Free(GA1-H4113) is expressed from E. coli cells. It contains AA Met 1 - Asp 135 (Accession # P09382-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Galectin-1 Structure

    This protein carries no "tag".

    The protein has a calculated MW of 14.71 kDa. The protein migrates as 13-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

    The protein is designed as a dimer.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Galectin-1 SDS-PAGE

Human Galectin-1, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Galectin-1 MALS images

The purity of Human Galectin-1, Tag Free (Cat. No. GA1-H4113) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Bioactivity-BLI
 Galectin-1 BLI

Loaded Human CD45, His Tag (Cat. No. CD5-H52H8) on HIS1K Biosensor, can bind Human Galectin-1, Tag Free (Cat. No. GA1-H4113) with an affinity constant of 3.0 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

 Galectin-1 BLI

Loaded Biotinylated Human CD45, His,Avitag (Cat. No. CD5-H82E7) on SA Biosensor, can bind Human Galectin-1, Tag Free (Cat. No. GA1-H4113) with an affinity constant of 2.0 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Galectin-1 (Gal-1), a member of the β-galactoside-binding animal lectin family, has a wide range of biological activities, which makes it an attractive target for medical applications. Gal-1 is ubiquitously but differentially expressed in normal and pathological animal tissues, and a variety of biological activities, including modulation of innate and adaptive immune responses, malignant progression of tumor cells, etc.
  • Clinical and Translational Updates

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  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Approved

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