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The kit is developed for quantitative detection of natural and recombinant human IL-8 in serum, plasma and cell culture supernatants.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | IL-8 |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Human |
| Sensitivity | 3.0 pg/mL |
| Assay Time | 1.75 hr |
| Sample volume | 50 uL |
| Range | 6.25 pg/mL-400 pg/mL |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| NIBSC Code | 89/520 |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CEA-C004 is less than 3.0 pg/mL.
Three human serum samples with high concentrations of IL-8 were diluted 1:2, 1:4, 1:8, 1:16, 1:32 with Dilution Buffer to produce samples with values within the dynamic range and then assayed. On average, 103.12% of IL-8 was detected from serum samples.
Ten replicates of each of five samples containing different IL-8 concentrations were tested in one assay , Intra-Assay Precision CV＜10%.
Five samples containing different concentrations of IL-8 were tested in three independent assays , Inter-Assay Precision CV<15%.
Recombinant IL-8 was spiked into 5 human serum samples, and then analyzed. On average, 97.54% of IL-8 was recovered from serum samples.
Recombinant IL-8 was spiked into cell supernatant sample, and then analyzed. On average, 98.87% of IL-8 was recovered from serum samples.
Bilirubin (simulated jaundice) concentration should be less than 20mg/dL, hemoglobin (simulated hemolysis) concentration should not be higher than 3500mg/dL, triglyceride (simulated lipemia) concentration may not be higher than 2.0g/L, Heparin concentration should be less than 40U/mL, EDTA concentration should be less than 4mg/mL, and Sodium citrate concentration may not be higher than 40mg/mL, it does not affect the detection result.
| ID | Components | Size |
| CEA004-C01 | Pre-coated Anti-IL-8 Antibody Microplate | 1 plate |
| CEA004-C02 | IL-8 Calibrator | 20 μg×2 |
| CEA004-C03 | Biotin-Anti-IL-8 Antibody Concentrated Solution | 100 μL |
| CEA004-C04 | Biotin Antibody Dilution Buffer | 8 mL |
| CEA004-C05 | IL-8 Streptavidin-HRP Concentrated Solution | 0.5 mL |
| CEA004-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA004-C07 | 20× Washing Buffer | 50 mL |
| CEA004-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA004-C09 | Substrate Solution | 12 mL |
| CEA004-C10 | Stop Solution | 6 mL |
Price(USD) : $549.00
Price(USD) :
Price(USD) :
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