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Rat ITGAV&ITGB3 Heterodimer, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB3, has a calculated MW of 112.3 kDa (ITGAV) & 81.6 kDa (ITGB3). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The protein migrates as 125-145 kDa (ITGAV) and 70-85 kDa (ITGB3) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Rat ITGAV&ITGB3 Heterodimer, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Rat ITGAV&ITGB3 Heterodimer, His Tag&Tag Free (Cat. No. IT3-R52E2) is more than 85% and the molecular weight of this protein is around 210-240 kDa verified by SEC-MALS.
Immobilized Human Vitronectin at 5 μg/mL (100 μL/well) can bind Rat ITGAV&ITGB3 Heterodimer, His Tag&Tag Free (Cat. No. IT3-R52E2) with a linear range of 0.039-1.25 μg/mL (QC tested).
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