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| ID | Components | Size |
| EP138-C01 | High-bind Plate | 1 plate |
| EP138-C02 | Human SIRP alpha | 15 μg |
| EP138-C03 | Biotinylated Human CD47 | 10 μg |
| EP138-C04 | Anti-CD47 Neutralizing Antibody | 20 μg |
| EP138-C05 | Streptavidin-HRP | 10 μg |
| EP138-C06 | Coating Buffer | 12 mL |
| EP138-C07 | 10xWashing Buffer | 50 mL |
| EP138-C08 | Blocking Buffer | 50 mL |
| EP138-C09 | Substrate Solution | 12 mL |
| EP138-C10 | Stop Solution | 12 mL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
INHIBITION OF CD47[Biotinylated]: SIRP alpha BINDING BY ANTI-CD47 NEUTRALIZING AN-TIBODY
Serial dilutions of Anti-CD47 Neutralizing antibody (Catalog # EP138-C04) (1:1 serial dilution, from 10 μg/mL to 0.01μg/mL) was added into Biotinylated CD47: SIRP alpha binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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