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Your Position: Home > Protein > Laminin 521 > LA5-H5261

Human Laminin 521 Protein, premium grade DMF

GMP version GMP-LA5H24 is now available for seamless transition.

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  • Product Overview
    ACRO offers three premium-grade Laminin 521 proteins, LA5-H5243 (Flagship Product) and LA5-H5213L & LA5-H5261 (Complementary Product), designed for iPSC cell expansion and differentiation applications. While both proteins undergo similar manufacturing processes with minor procedural variations, they are strategically positioned to address distinct user needs.

    Primary Recommendation: LA5-H5243 (Flagship Product)
    As the cornerstone of our laminin 521 protein portfolio, the Human Laminin 521 Flagship Protein (Premium Grade, Cat. No. LA5-H5243) stands as the optimal choice for new users and standardized workflows. Renowned for its superior activity in stem cell expansion, this protein also facilitates easier detachment during stem cell passage-qualities that make it the premier option for initial testing and implementation.

    Complementary Option: LA5-H5213L and LA5-H5261
    For some specialized applications requiring nuanced optimization, Human Laminin 521 Protein, premium grade (Cat. No. LA5-H5213L and LA5-H5261) may demonstrate marginally enhanced effects in specific experimental contexts. We recommend evaluating this product:
    1. If LA5-H5243 does not meet performance expectations in your system.
    2. During process development, parallel screening of both variants could identify the optimal fit for your workflow.
  • Product Details
    Laminin 521 (LN521) is a recombinant human protein that provides a defined surface for in vitro feeder-free culture of multiple human pluripotent stem cells (PSCs). LN521 has been proven to maintain normal growth characteristics and stemness in multiple PSC lines without simultaneous differentiation, which includes ESC, iPSC, MSC etc. In addition, LN521 has been demonstrated to support PSC growth for >10 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages.
    Laminin 521 protein premium grade (LA5-H5261) has the equivalent GMP version (GMP-LA5H24) with exactly the same performance, which enables a seamless transition from preclinical development to clinical phases.

    Flexible & Compatible
    Laminin 521 could work well in any commercial stem cell media. Meanwhile, it could support the attachment and expansion of hPSCs.

    Stemness maintenance
    Laminin 521 is the biologically relevant hPSCs extracellular matrix. It is crucial for the growth and stemness maintenance of hPSCs in human through its binding to cell receptors a6β1 integrin.

    Enhance cell differentiation
    Due to the diverse biorelevant environment, laminin 521 could also enhance cell differentiation, polarization and organization of target cell types.

    Reduce Variability
    LN521 is a defined, recombinant human protein with better lot-to-lot consistency that reduces variability in your PSC cultures.
  • Key parameter
    Purity (SDS PAGE)> 95%
    Mycoplasma TestNegative
    Sterility TestNegative
    Integrin Binding Assay0.5 nM < KD < 5 nM
    Endotoxin Test< 0.01 EU per μg
    Host Cell Protein< 0.5 ng/µg
    Host Cell DNA< 0.02 ng/μg
  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution;
    3. 2-8°C for 3 months under sterile conditions after reconstitution.
The schematic diagram of Laminin in ES/iPS cell culture
Bioactivity-Stem Cell Culture
 Laminin 521 STEM CELL CULTURE

Laminin 521 (LA5-H5261) could maintain the stemness of iPSC at least Passage 5. Immunofluorescent staining indicated that the iPSCs expressed high levels of pluripotency associated markers Sox2, TRA-1-60, SSEA4 and OCT4.

 Laminin 521 STEM CELL CULTURE

Laminin 521 (LA5-H5261) could maintain the stemness of iPSC at least Passage 5. FACS data indicated that the iPSCs expressed high levels of pluripotency associated markers OCT4 and SOX2.

 Laminin 521 STEM CELL CULTURE

Normal karyotype (46, XX) was found in hiPSCs with Laminin 521 (LA5-H5261) coating after 10 passages.

Bioactivity-BLI
 Laminin 521 BLI

Loaded Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) on HIS1K Biosensor, can bind Human Laminin 521 Protein, premium grade (Cat. No. LA5-H5261) with an affinity constant between 0.50 nM - 5.00 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Bioactivity-Stability
 Laminin 521 STABILITY

The BLI based assay shows batch-to-batch consistency between Acro's GMP and PG Laminin 521.

  • Clinical and Translational Updates

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