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| ID | Components | Size |
| EP146-C01 | High-bind Plate | 1 plate |
| EP146-C02 | Human IL-6 R alpha | 15 μg |
| EP146-C03 | Biotinylated Human IL-6 | 10 μg |
| EP146-C04 | Anti-IL-6 Neutralizing Antibody | 10 μg |
| EP146-C05 | Streptavidin-HRP | 10 μg |
| EP146-C06 | Coating Buffer | 12 mL |
| EP146-C07 | 10xWashing Buffer | 50 mL |
| EP146-C08 | Blocking Buffer | 50 mL |
| EP146-C09 | Substrate Solution | 12 mL |
| EP146-C10 | Stop Solution | 7 mL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
INHIBITION OF IL-6 [Biotinylated]: IL-6 R alpha BINDING BY ANTI-IL6 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-IL-6 Neutralizing antibody (Catalog # EP146-C04) (1:1 serial dilution, from 10 μg/mL to 0.01μg/mL) was added into Biotinylated IL-6: IL-6 R alpha binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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