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Your Position: Home > Antibody > PD-L1 > HCS-S240

Recombinant Monoclonal Anti-PD-L1 Antibody, Rabbit (5D3)

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INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Background
    Recombinant Monoclonal Anti-PD-L1 Antibody, Rabbit (5D3) is a PD-L1 primary antibody for IHC application. PD-L1, is a ligand that binds with the receptor PD1, commonly found on T-cells, and acts to block T-cell activation. PD1 expression has been observed in a variety of cancers including melanoma and non-small cell lung cancer. The interaction of PD1/PDL1 is hypothesized to be a possible mechanism for the tumor to escape immune response. A number of checkpoint blockade inhibitors including pembrolizumab and nivolumab have been developed that target the PD1/PDL1 interaction in order to allow T-cells to recognize tumor cells without being deactivated by the tumor.
  • Key parameter
    Host SpeciesRabbit
    IsotypeIgG
    Clone5D3
    ApplicationIHC
    Property1：1000
    StateLiquid
    Positive ControlHuman Tonsil Tissues
    ClonalityMonoclonal
    Research FieldCancer Drug Targets
    SourceRabbit
    SynonymCD274, PD-L1
  • Experiment Protocol
    LEICA Experiment Protocol(Manual)
  • Storage
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. The shelf life is 30 days from the date of opening.
Typical Data
Control Sample
PD-L1 CONTROL SAMPLE

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with HCS-S240 at 1/1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Cancer Sample
PD-L1 CANCER SAMPLE PD-L1 CANCER SAMPLE PD-L1 CANCER SAMPLE

Immunohistochemical analysis of paraffin embedded human cancer tissue labelled with HCS-S240 at 1/1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Clinical and Translational Updates

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