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Your Position: Home > Antibody > Ki-67 > HGS-S239

Recombinant Monoclonal Anti-Ki-67 Antibody, Rabbit (7A4)

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  • Background
    Recombinant Monoclonal Anti-Ki-67 Antibody, Rabbit (7A4) is a Ki-67 primary antibody for IHC application. Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility.
  • Key parameter
    Host SpeciesRabbit
    IsotypeIgG
    Clone7A4
    ApplicationIHC
    Property1:500
    StateLiquid
    Positive ControlHuman Tonsil Tissues
    ClonalityMonoclonal
    Research FieldCancer Drug Targets
    SourceRabbit
    SynonymPPP1R105, MIB-1, ki-67
  • Experiment Protocol
  • Storage
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. The shelf life is 30 days from the date of opening.
Typical Data
Control Sample
 Ki-67 CONTROL SAMPLE

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with HGS-S239 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Cancer Sample
 Ki-67 CANCER SAMPLE Ki-67 CANCER SAMPLE

Immunohistochemical analysis of paraffin embedded human cancer tissue labelled with HGS-S239 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Clinical and Translational Updates

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