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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 56.3 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, 150 mM NaCl, pH5.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
HRSV (B) Pre-fusion glycoprotein F0, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) is more than 90% and the molecular weight of this protein is around 190-240 kDa verified by SEC-MALS.
Immobilized HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) at 1 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (D25) with a linear range of 0.008-0.25 μg/mL (QC tested).
Immobilized Recombinant Human Anti-RSV Antibody (AM14), Human IgG1 at 1 μg/mL (100 μL/well) can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with a linear range of 0.5-31 ng/mL (QC tested).
Immobilized HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) at 1 μg/mL (100 μL/well) can bind Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) with a linear range of 0.1-4 ng/mL (Routinely tested).
Immobilized Nirsevimab, Human IgG1 kappa at 1 μg/mL (100 μL/well) can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with a linear range of 0.2-16 ng/mL (Routinely tested).
Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) captured on Protein G-Series S sensor chip can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with an affinity constant of 0.766 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Monoclonal Anti-HRSV-A F/Fusion glycoprotein F0 Antibody, Human IgG1 (D25) captured on Protein G-Series S sensor chip can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with an affinity constant of 0.792 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Monoclonal Anti-RSV-F0 broadly Antibody, Mouse IgG1 (4G2) captured on Protein G-Series S sensor chip can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with an affinity constant of 6.69 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Monoclonal Anti-RSV-Pre-F0 specific Antibody, Human IgG1 (12C6) (Cat. No. RS0-S286) captured on Protein G-Series S sensor chip can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with an affinity constant of 2.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Monoclonal Anti-RSV-F0 broadly Antibody, Mouse IgG1 (3B4) captured on CM5 chip via anti-mouse antibodies surface can bind HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8) with an affinity constant of 2.58 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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