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• Serum free adapted suspension HEK 293 transient expression
• Adherent HEK 293 transient expression (both serum contained and serum free)
• HEK293 based transient expression (both serum contained and serum free, or adherent and suspension culture)
Proprietary components in PBS buffer, pH 8.0. Sterile for cell culture use.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
• Liquid solution and stable in -20ºC for 1 year
• Frequent freeze-thaw cycle should be avoid
• Aliquot the reagent into smaller quantities for optimal storage is recommended.
Add appropriate volume of 293 Expression MAX-1 to solution of plasmid containing your target gene before transfection reagent addition.
Mixing for 10 second by vortex.
Normally, dosage of 40uL 293 Expression MAX-1 per 100ug plasmid is recommended for transient expression, however, it can be optimized to specific to your experiment from 5uL to 50uL per 100ug plasmid to get optimal result.
Comparision Result for Human IgG1 Fc TGE with or without 293 Expression MAX-1 Enhancer:
Serum free adapted HEK293 cell line was employed in transient expression experiment. Compared with the transfection without 293 Expression MAX-1, the expression titer of human IgG1 Fc protein was over tripled fold increased when the 293 Expression MAX-1 reagent was applied(40uL reagent per 100 ug TGE plasmid).
The whole process is in animal origin free and chemically defined cell culture medium, without adding any growth factor or hydrolysate supplementation.
| Cat. No. | Product Name | Quantity | Storage Conditions |
|---|---|---|---|
| TF-1157-11 | BPfectin | 1.5 mL | -20°C |
| EXP-711-11 | 293 Expression MAX-1 | 1 mL | -20°C |
Emerging VoCs, Omicron, Delta, Beta, Alpha mutants and so on, including RBD, S trimer, S1, NTD, NP, etc. These mutants are of high purity and bioactivity and can be used to evaluate the efficacy of the antibodies and vaccination.
ACROBiosystems developed a series of GMP grade cytokines under the GMP grade quality management system. Those products are all suitable for T/NK cell generation, activation, and proliferation in cell therapy research.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
CD3 proteins and a collection of for bispecific antibody development which are of high specificity and bioactivities and suitable for immunization, antibody screening.
A series of immune checkpoints including classic co-inhibitory and co-stimulatory receptors. The comprehensive catalog contains 100+ targets with various species and tags, and the high-quality proteins are in good batch-to-batch consistency.
To meet the needs of ADCs development, ACROBiosystems can provide: A variety of high-quality target proteins; MMPs/Cathepsin/uPA for cleavable linker; Anti-payload antibodies & anti-idiotypic antibodies for immunogenicity and PK analysis; SPR/BLI analytical and ADA development service.
Comprehensive collection of Fc receptor proteins, including their common variants, which can help expedite your antibody development.
The MABSOL biotinylated protein collection includes more than a hundred commonly studied drug targets and biomarker proteins.
Comprehensive cytokines including IL families, growth factors, chemokines, TNFs, etc. These products are HEK293 expressed and nearly in authentic structure, high purity and bioactivity, cell based assay/SPR/BLI verified.
To support preclinical/clinical immunogenicity and PK analysis, ACROBiosystems has developed a series of high-affinity anti-idiotypic antibodies. Our pipeline covers five hot targets including adalimum*b, rituxim*b, cetuxim*b, trastuzum*b, and bevacizum*b.
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