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Human OX40 (Luc) HEK293 Reporter Cell

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  1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
  2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
  3. Comprehensive application data to support assay development and validation
  4. Full tracible record, stringent quality control and validated cell passage stability
  5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
  6. Global commercial license assistance whenever regulatory filing is required
  • Description
    The Human OX40 (Luc) HEK293 Reporter Cell was engineered to not only express NF-κB signaling response element, but also express the receptor full length human OX40 (Uniprot: P43489), which can drive luciferase expressing systems by OX40 ligand/agonist antibody stimulation. When stimulated with human OX40 ligand protein, the OX40 ligand/OX40 interaction drives NF-κB-mediated luminescence. Inhibition of OX40 ligand binding to OX40 by either anti-OX40 ligand or anti-OX40 antibodies results in a decrease in luminescence.
  • Application

    • Screen for anti-human OX40 ligand or anti-human OX40 neutralizing antibody.

    • Screen for ligands or agonist antibodies that can bind and activate OX40.

     OX40 Assay Principles

  • Growth Properties
    Adherent
  • Selection Marker
    Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL)
  • Complete Growth Medium
    DMEM + 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.

Receptor Assay
 OX40 FACS

Expression analysis of human OX40 on Human OX40 (Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human OX40 (Luc) HEK293 Reporter Cell or negative control cell using biotinylated human OX40 ligand protein followed by staining with Streptavidin-PE.

Application
 OX40 APPLICATION

Inhibition of human OX40 ligand protein-induced reporter activity by anti-human OX40 ligand neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human OX40 ligand protein with a final concentration of 0.02 μg/mL. The EC50 of anti-human OX40 ligand neutralizing antibody (Amlitelimab) was approximately 0.025 μg/mL.

Signaling Bioassay
 OX40 SIGNALING

Response to human OX40 ligand protein (RLU).
The Human OX40 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human OX40 ligand protein. The EC50 was approximately 0.0106 μg/mL.

 OX40 SIGNALING

Response to human OX40 ligand protein (FOLD).
The Human OX40 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human OX40 ligand protein. The max induction fold was approximately 12.04.

Please contact us if you are interested in related cell pool service.

  • Limited Use&License Disclosure

    BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE FOLLOWING TERMS OF LIMITED USE OF THIS CELL LINE PRODUCT.

    1. If the researcher is not willing to accept the terms of limited use of this cell line product, and the product is unused, ACRO will accept return of the unused product.
    2. Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for profit or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research.
    3. This cell line is neither intended for any animal or human therapeutic purposes nor for any direct human in vivo use . You have no right to share, modify, transfer, distribute, sell, sublicense, or otherwise make the cell line available for use to other researchers, laboratories, research institutions, hospitals, universities, or service organizations.
    4. ACROBIOSYSTEMS MAKES NO WARRANTIES OR REPRESENTATIONS OF ANY KIND, EITHER EXPRESSED OR IMPLIED, WITH RESPECT TO THE SUITABILITY OF THE CELL LINE FOR ANY PARTICULAR USE.
    5. ACROBIOSYSTEMS ACCEPTS NO LIABILITY IN CONNECTION WITH THE HANDLING OR USE OF THE CELL LINE.
    6. Modifications of the cell line, transfer to a third party, or commercial use of the cell line may require a separate license and additional fees. Please contact order@acrobiosystems.com for further details.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $13150.00

Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:27 Details
  • Latest Research Phase:Phase 3 Clinical

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