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Your Position: Home > Protein > IgG4 Fc > IG4-H5205

Human IgG4 Fc Protein, Tag Free (MALS & SPR verified)

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  • Synonym
    IGHG4
  • Source
    Human IgG4 Fc, Tag Free(IG4-H5205) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 327 (Accession # P01861).
    Predicted N-terminus: Glu 99
  • Molecular Characterization
    IgG4 Fc Structure

    This protein carries no "tag".

    The protein has a calculated MW of 25.8 kDa. The protein migrates as 30-33 kDa under reducing (R) condition, and 29 kDa and 55-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IgG4 Fc SDS-PAGE

Human IgG4 Fc, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IgG4 Fc MALS images

The purity of Human IgG4 Fc, Tag Free (Cat. No. IG4-H5205) is more than 95% and the molecular weight of this protein is around 54-66 kDa verified by SEC-MALS.

Bioactivity-SPR
 IgG4 Fc SPR

Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG4 Fc, Tag Free (Cat. No. IG4-H5205) with an affinity constant of 0.715 μM as determined in SPR assay (Biacore 8K) (QC tested).

  • Background
    Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
  • Clinical and Translational Updates

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