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The kit is developed for quantitative detection of natural and recombinant human IL-17A / CTLA8 in serum, plasma and cell culture supernatants.
It is for research use only.
| Analyte | IL-17A/CTLA8 |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | <6pg/mL |
| Range | 31.25 pg/mL-2000 pg/ml |
| Assay Time | 2hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 μL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-17A is less than 6 pg/mL.
3 types of materials were tested to observe if there were matrix effect (interference). If the concentration of hemoglobin (simulated hemolysis) is less than 3500 mg/dL(2%), the concentration of triglyceride (simulated lipid blood) is less than 3 mg/mL, testing results will not be affected.
High concentrations of human IL-17A serum samples were diluted with 1:2, 1:4, 1:8 and 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of IL-17A was 90.14%.
Ten replicates of each of 4 samples containing different IL-17A concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Five samples containing different concentrations of IL-17A were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant IL-17A was spiked into 3 human serum samples, and then analyzed. The average recovery of IL-17A for serum samples is 85.37%.
| ID | Components | Size |
| CEA092-C01 | Pre-coated Anti-IL-17A Antibody Microplate | 1 plate |
| CEA092-C02 | Human IL-17A Standard | 20μg ×2 |
| CEA092-C03 | Biotin-Anti-IL-17A Antibody Con. Solution | 300 μL |
| CEA092-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA092-C05 | Streptavidin-HRP Con. Solution | 500 uL |
| CEA092-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA092-C07 | 20× Washing Buffer | 50 mL |
| CEA092-C08 | Sample Dilution Buffer | 15 mL ×2 |
| CEA092-C09 | Substrate Solution | 12 mL |
| CEA092-C10 | Stop Solution | 6 mL |
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