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This protein carries no "tag".
The protein has a calculated MW of 13.8 kDa.
>95% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in PBS, pH7.4.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Tau-441 K18 (P301L) monomer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Transmission electron microscopy (TEM) of Human Tau-441 K18 (P301L) Pre-formed Fibrils Protein, Tag Free (Cat. No. TAU-H5113). Fibril structure is visible on negative stain TEM images of TAU-H5113 (Routinely tested).
Human Tau-441 K18 (P301L) Pre-formed Fibrils Protein, Tag Free (Cat. No. TAU-H5113) induce tau phosphorylation and astrocyte activation in brain organoids (DIV100+, CIPO-BWL002K).
HEK293/Human Tau (GFP) Stable Cell Line (Cat. No. CHEK-ATP087) were transduced with Human Tau-441 K18 (P301L) Pre-formed Fibrils Protein, Tag Free (Cat. No. TAU-H5113). The fluorescence of GFP-Tau (Green) were detected by confocal microscope. A. Lipo2000 transduction. B. Lipo2000 and Human Tau-441 K18 (P301L) Pre-formed Fibrils Protein , Tag Free (Cat. No. TAU-H5113) transduction. C. Score calculated from spot number/cell number. Both PEI and Lipo2000 can help tau-pFFs transfection and 4ug/ml is a better concentration when transfect cells in 24 well plate. Scale bars, 100 μm.
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