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Your Position: Home > Kits > IL-7 > RES-A055

resDetect™ Human Interleukin-7 (IL-7) ELISA Kit (Residue Testing)

For research use only.

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    Product Specifications
    Assay TypeSandwich-ELISA
    AnalyteIL-7
    Format96T(8×12 strips)
    ReactivityHuman
    Regulatory StatusRUO
    Sensitivity<6.25pg/mL
    Standard Curve Range6.25 pg/mL-200 pg/mL
    Assay Time2 hr 50 min
    Suitable Sample TypeFor the quantitative determination of human IL-7 in Cell Culture Supernatants, Plasma, Serum.
    Sample volume100 uL
  • Product Overview
    resDetect™ Human Interleukin-7 (IL-7) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IL-7 residues in cell culture supernatants, serum, and plasma. The kit contains GMP human IL-7 (ACROBiosystems, cat# GMP-L07H24) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human Interleukin-7 (IL-7).
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Materials Provided
    IDComponentsSize
    RES055-C01Pre-coated Anti-IL-7 Antibody Microplate1 plate(8×12 strips)
    RES055-C02Human IL-7 Standard20 μg
    RES055-C03Biotin-Anti-IL-7 Antibody20 μg
    RES055-C04Streptavidin-HRP50 μL
    RES055-C0510xWashing Buffer 50 mL
    RES055-C062xDilution Buffer50 mL
    RES055-C07Substrate Solution12 mL
    RES055-C08Stop Solution7 mL
Typical Data Please refer to DS document for the assay protocol.
 IL-7 TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

  • Background: IL-7
    IL7, Interleukin-7
    Interleukin 7 is also known as IL7, IL-7, and is a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells, but is not produced by lymphocytes. IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells, It also stimulates proliferation of all cells in the lymphoid lineage (B cells, T cells and NK cells). It is important for proliferation during certain stages of B-cell maturation, T and NK cell survival, development and homeostasis. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL-7 binds to the IL-7 receptor, a heterodimer consisting of Interleukin-7 receptor alpha and common gamma chain receptor. IL-7 promotes hematological malignacies (acute lymphoblastic leukemia, T cell lymphoma). Elevated levels of IL-7 have also been detected in the plasma of HIV-infected patients. IL-7 as an immunotherapy agent has been examined in many pre-clinical animal studies and more recently in human clinical trials for various malignancies and during HIV infection. IL-7 could also be beneficial in improving immune recovery after allogenic stem cell transplant.
  • Clinical and Translational Updates

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ETA des produits en stock: 2 jours ouvrables

Price(USD) : $348.00

Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Phase 1 Clinical

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