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After recovery, please store the organoid in its maintenance medium under the correct incubation condition and medium changing process.
Protocol Diagram of liver organoid differentiation.
H&E Staining of liver organoid.
The liver organoids show expression of hepatocytes (HNF4a), hepatobiliary tracts (CK19), Albumin (ALB) and vascular endothelial cell (CD31).
Pre-mature liver organoids show liver lobules-like structure.
Liver organoids show better performance than HepG2 cell line in terms of the expression of Albumin, CYP3A4, CYP1A2, CYP2C9, CYP7A1 and BSEP.
Liver organoid show response to Rifampicin treatment as indication by the increase fluorescence intensity of CYP3A4.
At 20 days of age, the albumin secretion of ACRO's liver organoids was consistent with the secretion amount reported in the literature, being 15 pg Albumin / ng RNA. As the organoids continued to mature and grow, their albumin secretion would keep increasing, reaching a level slightly lower than that of the primary tissue.
ACRO utilized liver organoids to construct the NASH model. As can be seen from the oil red staining results shown in the above figure, the modeling group of NASH exhibited obvious lipid accumulation (red positive staining).
During the process of constructing the NASH model, we detected the expression of inflammatory factors in liver organoids through qPCR experiments, and observed the upregulation of factors such as IL-6 and IL-33. This confirmed the inflammatory response of our liver organoids.
Liver organoids can be used to detect hepatotoxicity. After treating liver organoids with the commonly hepatotoxic positive drug Trogalitazone, changes in cell viability within the liver organoids can be observed through the results of AO/PI staining.
Fluorescent-free 5-Carboxy-2',7'-Dichlorofluorescein with organoesterase activity are metabolized into fluorescent-emitting CDF, and CDF is actively transported from hepatocytes to the bile duct network and deposited in the bile duct network to simulate the cholestasis process. Trogalitazone treated organoids shows the accumulation of the CDF.
Emerging VoCs, Omicron, Delta, Beta, Alpha mutants and so on, including RBD, S trimer, S1, NTD, NP, etc. These mutants are of high purity and bioactivity and can be used to evaluate the efficacy of the antibodies and vaccination.
ACROBiosystems developed a series of GMP grade cytokines under the GMP grade quality management system. Those products are all suitable for T/NK cell generation, activation, and proliferation in cell therapy research.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
CD3 proteins and a collection of for bispecific antibody development which are of high specificity and bioactivities and suitable for immunization, antibody screening.
A series of immune checkpoints including classic co-inhibitory and co-stimulatory receptors. The comprehensive catalog contains 100+ targets with various species and tags, and the high-quality proteins are in good batch-to-batch consistency.
To meet the needs of ADCs development, ACROBiosystems can provide: A variety of high-quality target proteins; MMPs/Cathepsin/uPA for cleavable linker; Anti-payload antibodies & anti-idiotypic antibodies for immunogenicity and PK analysis; SPR/BLI analytical and ADA development service.
Comprehensive collection of Fc receptor proteins, including their common variants, which can help expedite your antibody development.
The MABSOL biotinylated protein collection includes more than a hundred commonly studied drug targets and biomarker proteins.
Comprehensive cytokines including IL families, growth factors, chemokines, TNFs, etc. These products are HEK293 expressed and nearly in authentic structure, high purity and bioactivity, cell based assay/SPR/BLI verified.
To support preclinical/clinical immunogenicity and PK analysis, ACROBiosystems has developed a series of high-affinity anti-idiotypic antibodies. Our pipeline covers five hot targets including adalimum*b, rituxim*b, cetuxim*b, trastuzum*b, and bevacizum*b.
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