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Monoclonal Anti-Human Phospho-Tau (Thr181) Antibody, Mouse IgG2a (8D8C10) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
8D8C10
Mouse
Mouse IgG2a | Mouse Kappa
Unconjugated
Hybridoma Monoclonal
Human
Recombinant polypeptide protein
Specifically recognizes Human p-tau181 Protein.
| Application | Recommended Usage |
| IF | 1:50-1:500 |
>95% as determined by SDS-PAGE.
Protein A purified / Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Monoclonal Anti-Human Phospho-Tau (Thr181) Antibody, Mouse IgG2a (8D8C10) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
2D cell staining: Immunofluorescent staining (10X) of phosphorylated tau in treated SH-SY5Y neuroblastoma cells with purified PT1-Y2073 at 1:200 dilution. DAPI (blue) was used as nuclear counterstain.
Detection of Monoclonal Anti-Human p-tau181 antibody,Mouse,Mouse IgG2a（8D8C10), Mouse IgG2a | Kappa,HEK by Western Blot. Monoclonal Anti-Human p-tau181 antibody,Mouse,Mouse IgG2a（8D8C10), Mouse IgG2a | Kappa,HEK at 10.0 ug/ml dilution + Phosphorylated SH-SY5Y cell lysate, Mouse Tag at 20uL.
Secondary: (MFC)-Peroxidase AffiniPure GoatAnti-MouseIgG,FcγFragmentSpecific(1:1500).
Predicted band size: 65 kDa 12% Bis-Tris Protein Gel.
The recommended dilution ratio is 10.0 ug/ml.
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