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Your Position: Home > Cell Grade Beads > CD229 > MBS-C010

ActiveMax® Human CD229 μBeads, premium grade (for cells)

The Magnetic Stand (Cat.No. MB-01 & Cat.No. MB-02) can be used in conjunction with Beads.

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ProductSizeAmount
ActiveMax® Human CD229 μBeads, premium grade (for cells)2.5 mg2.5×10⁷ beads
  • Product Description
    ActiveMax® Human CD229 μBeads, premium grade (for cells) are uniform, superparamagnetic beads of 5.5 µm in diameter immobilized with Human CD229 / SLAMF3 protein expressed from human 293 cells (HEK293) and contains AA Lys 48 - Lys 454 (Accession # Q9HBG7-1).
    ActiveMax® Human CD229 μBeads, premium grade (for cells) are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.
  • Application
    ActiveMax® Human CD229 μBeads, premium grade (for cells) are designed to stimulate in vitro CD229-specific CAR-T cells or UCAR-T cells, similar to the tumor cell lines that express human CD229 antigen. It can be used as follows:
    Evaluating the characteristics of CD229-specific CAR-T cells or UCAR-T cells.
    In vitro expansion of CD229-specific CAR-T cells or UCAR-T cells.
    In vitro enrichment of CD229-specific CAR-T cells or UCAR-T cells.
  • Reconstitution

    See Certificate of Analysis (CoA) for detailed instruction.

  • Storage
    This product is stable in storage under the following conditions: -20˚C for 12 months in lyophilized state. -70°C for 3 months under sterile conditions after reconstitution.Please avoid repeated freeze-thaw cycles after reconstitution. Immediate use after reconstitution is highly recommended.
  • Sterility
    Negative
  • Endotoxin
    Less than 0.002 EU per μg by the LAL method / rFC method.
  • Important Note
    This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
  • Formulation

    Please contact us for detailed information.

    Contact us for customized product form or formulation.

FACS Data
 CD229 FACS

Assay of human CD229 protein on the μBeads surface by Flow cytometry. The human CD229 protein conjugated on the μBeads (Cat. No. MBS-C010) surface were fluorescently stained using PE anti-human CD229 Antibody and analyzed by flow cytometry.

Application Data
Stimulate CD229 CAR-T Cells -Cytokines
 CD229 CYTOKINES

CD229 CAR-T cells were stimulated with ActiveMax® Human CD229 μBeads (MBS-C010) for 24 h , and cell-free supernatants were harvested for evaluating IFN-γ、TNF-α、IL-4、IL-6 secretion by flow cytomtry. The results showed that CD229 CAR-T cells released significantly larger amounts of IFN-γ、TNF-α、IL-4、IL-6 into the supernatants in response to CD229 μBeads.

Stimulate CD229 CAR-T Cells -Cytotoxic Molecules
 CD229 CYTOTOXIC MOLECULES

CD229 CAR-T cells were stimulated with ActiveMax® Human CD229 μBeads (MBS-C010) for 24h, and cell-free supernatants were harvested for evaluating Granzyme B secretion by ELISA. The results showed that CD229 CAR-T cells released significantly larger amounts of Granzyme B into the supernatants in response to CD229 μBeads.

  • Clinical and Translational Updates

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