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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.5 kDa. The protein migrates as 23-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) at 5 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Human IgG1 with a linear range of 0.2-16 ng/mL (Routinely tested).
Immobilized Anti-CD8 alpha Antibody, Human IgG1 at 5 μg/mL (100 μL/well) can bind Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) with a linear range of 1-16 ng/mL (Routinely tested).
1e5 of Mouse Anti-CD8 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
1e5 of Mouse Anti-CD8 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag (Cat. No. CDA-HA2H6) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Binding activity of three different lots of Alexa Fluor 488-Labeled Human CD8 alpha Protein, His Tag against Mouse Anti-CD8 antibody coupled beads (5.5 μm) was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.
Emerging VoCs, Omicron, Delta, Beta, Alpha mutants and so on, including RBD, S trimer, S1, NTD, NP, etc. These mutants are of high purity and bioactivity and can be used to evaluate the efficacy of the antibodies and vaccination.
ACROBiosystems developed a series of GMP grade cytokines under the GMP grade quality management system. Those products are all suitable for T/NK cell generation, activation, and proliferation in cell therapy research.
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GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
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A series of immune checkpoints including classic co-inhibitory and co-stimulatory receptors. The comprehensive catalog contains 100+ targets with various species and tags, and the high-quality proteins are in good batch-to-batch consistency.
To meet the needs of ADCs development, ACROBiosystems can provide: A variety of high-quality target proteins; MMPs/Cathepsin/uPA for cleavable linker; Anti-payload antibodies & anti-idiotypic antibodies for immunogenicity and PK analysis; SPR/BLI analytical and ADA development service.
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The MABSOL biotinylated protein collection includes more than a hundred commonly studied drug targets and biomarker proteins.
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To support preclinical/clinical immunogenicity and PK analysis, ACROBiosystems has developed a series of high-affinity anti-idiotypic antibodies. Our pipeline covers five hot targets including adalimum*b, rituxim*b, cetuxim*b, trastuzum*b, and bevacizum*b.
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