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| Assay Type | Sandwich-ELISA |
| Analyte | Laminin 521 |
| Format | 96T (8×12 strips) |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | ＜25 pg/mL |
| Standard Curve Range | 25 pg/mL-800 pg/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the detection and quantitative determination of Laminin 521 in human serum and cell culture supernates. |
| Sample volume | 100 μL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| RES040-C01 | Pre-coated Anti-Laminin 521 Antibody Microplate | 1 plate (8×12 strips) |
| RES040-C02 | Human Laminin 521 Standard | 20 μg |
| RES040-C03 | Biotin-Anti-Laminin Antibody | 20 μg |
| RES040-C04 | Streptavidin-HRP | 50 μL |
| RES040-C05 | 10×Washing Buffer | 50 mL |
| RES040-C06 | 2×Dilution Buffer | 50 mL |
| RES040-C07 | Substrate Solution | 12 mL |
| RES040-C08 | Stop Solution | 7 mL |
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
To assess the linearity of the assay, samples spiked with high concentrations of Laminin 521 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.
Three Laminin 521 with different concentrations were tested to calculate the recovery rate.
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