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Your Position: Home > Kits > Fc epsilon RI alpha > EP-169

Fc epsilon RI alpha [Biotinylated] : IgE Fc Inhibitor Screening ELISA Kit

For research use only.

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    Materials Provided
    IDComponentsSize
    EP169-C01High-bind Plate1 plate
    EP169-C02Human IgE Fc60 μg
    EP169-C03Human Fc epsilon RI alpha-SABC0.583 μg
    EP169-C04Anti-IgE (Omalizumab) 30 μg
    EP169-C05Coating Buffer12 mL
    EP169-C0620×Washing Buffer 50 mL
    EP169-C07Blocking Buffer50 mL
    EP169-C08Substrate Solution12 mL
    EP169-C09Stop Solution7 mL
  • Product Overview
    Fc epsilon RI alpha [Biotinylated] : IgE Fc Inhibitor Screening ELISA Kit is based on a competition-ELISA method, is used to screening for inhibitors of human Fc epsilon RI alpha binding to human IgE Fc. The kit is designed to provide a reliable solution for the screening of antibodies and small molecule inhibitors in the early stages, as well as QC release, to help researchers quickly screen and validate drugs, effectively improving research and development efficiency. It can also be used as a universal detection tool to identify the ability of human Fc epsilon RI alpha binding to human IgE Fc.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Typical Data Please refer to DS document for the assay protocol.
     Fc epsilon RI alpha TYPICAL DATA

    Serial dilutions of Anti-IgE (Omalizumab) (Catalog # EP169-C04) (1:1 serial dilution, from 5μg/mL to 0.078125μg/mL) was added into Human IgE Fc: Biotinylated Human Fc epsilon RI alpha binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The example data is for reference only.

    • Background: Fc epsilon RI alpha
      High affinity immunoglobulin epsilon receptor subunit alpha (FCER1A) is also known as Fc-epsilon RI-alpha (FcERI), IgE Fc receptor subunit alpha, FCE1A. FCER1A contains two Ig-like (immunoglobulin-like) domains. FCER1A binds to the Fc region of immunoglobulins epsilon and is a high affinity receptor. FCER1A is responsible for initiating the allergic response, which binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. FCER1A plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.
    • Clinical and Translational Updates

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    ETA des produits en stock: 2 jours ouvrables

    Drug Development Status

    • Number of Launched Drugs:1 Details
    • Number of Drugs in Clinical Trials:0 Details
    • Latest Research Phase:Approved

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