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Your Position: Home > Protein > GLP1R > GLR-H82D3

Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag™ (Detergent)

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  • Synonym
    GLP-1-R, GLP-1R, glucagon like peptide 1 receptor
  • Source
    Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag(GLR-H82D3) is expressed from human 293 cells (HEK293). It contains AA Arg 24 - Ser 432 (Accession # P43220-1).
    Predicted N-terminus: Asp
  • Molecular Characterization
    GLP1R Structure

    This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 67.9 kDa.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.
    DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
Bioactivity-ELISA
 GLP1R ELISA

Immobilized Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Glutazumab with a linear range of 0.06-2 ng/mL (QC tested).

 GLP1R ELISA

Immobilized Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Dulaglutide with a linear range of 0.5-31 ng/mL (Routinely tested).

Bioactivity-SPR
 GLP1R SPR

Dulaglutide captured on Protein A Chip can bind Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) with an affinity constant of 1.91 µM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).

 GLP1R SPR

Glutazumab captured on Protein A Chip can bind Biotinylated Human GLP1R Full Length Protein, Flag Tag&His,Avitag (Cat. No. GLR-H82D3) with an affinity constant of 30.6 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).

  • Background
    This gene encodes a 7-transmembrane protein that functions as a receptor for glucagon-like peptide 1 (GLP-1) hormone, which stimulates glucose-induced insulin secretion. This receptor, which functions at the cell surface, becomes internalized in response to GLP-1 and GLP-1 analogs, and it plays an important role in the signaling cascades leading to insulin secretion. It also displays neuroprotective effects in animal models. Polymorphisms in this gene are associated with diabetes. The protein is an important drug target for the treatment of type 2 diabetes and stroke.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:0 Details
  • Latest Research Phase:Preclinical

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