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Your Position: Home > Protein > DcR3 > DC3-H52H3

Human DcR3 / TNFRSF6B Protein, His Tag (MALS verified)

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  • Synonym
    TNFRSF6B,DCR3,TR6,M68
  • Source
    Human DcR3 Protein, His Tag(DC3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Ser 195 (Accession # O95407).
  • Molecular Characterization
    DcR3 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 20.1 kDa. The protein migrates as 27-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
DcR3 SDS-PAGE

Human DcR3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
DcR3 MALS images

The purity of Human DcR3 Protein, His Tag (Cat. No. DC3-H52H3) is more than 85% and the molecular weight of this protein is around 22-32 kDa verified by SEC-MALS.

Bioactivity-ELISA
 DcR3 ELISA

Immobilized Human DcR3 Protein, His Tag (Cat. No. DC3-H52H3) at 5 μg/mL (100 μL/well) can bind Human TL1A Protein, Fc Tag (Cat. No. TLA-H5263) with a linear range of 0.06-4 ng/mL (QC tested).

 DcR3 ELISA

Immobilized Human TL1A Protein, Fc Tag (Cat. No. TLA-H5263) at 5 μg/mL (100 μL/well) can bind Human DcR3 Protein, His Tag (Cat. No. DC3-H52H3) with a linear range of 1-31 ng/mL (Routinely tested).

Bioactivity-SPR
 DcR3 SPR

Human TL1A Protein, Fc Tag (Cat. No. TLA-H5263) captured on Protein A Chip can bind Human DcR3 Protein, His Tag (Cat. No. DC3-H52H3) with an affinity constant of 21.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B) is also known as Decoy Receptor 3 (DcR3), Decoy receptor for Fas ligand and M68, which belongs to the tumor necrosis factor receptor superfamily. TNFRSF6 is a soluble protein which contains four TNFR-Cys repeats. Unlike most of the other members of TNFR superfamily, TNFRSF6 is a soluble protein which contains no transmembrane domain. TNFRSF6B acts as a decoy receptor that competes with death receptors for ligand binding. The protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation as well as to induce angiogenesis via neutralization of TL1A.
  • Clinical and Translational Updates

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