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Your Position: Home > Protein > CD4 > CD4-HA2H8

Alexa Fluor™ 647-Labeled Human CD4 Protein, His TagStar Staining

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  • Synonym
    CD4,CD4mut,LEU3
  • Source
    Alexa Fluor 647-Labeled Human CD4 Protein, His Tag (CD4-HA2H8) is produced via conjugation of AF647 to Human CD4 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD4 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # AAH25782).
    Predicted N-terminus: Lys 26
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

  • Conjugate

    AF647

    Excitation Wavelength: 640 nm

    Emission Wavelength: 672 nm

  • Protein Ratio
    The AF647 to protein molar ratio is 0.9-1.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-ELISA
 CD4 ELISA

Immobilized Alexa Fluor 647-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA2H8) at 1 μg/mL (100 μL/well) can bind Anti-CD4 Antibody (Ibalizumab-IgG4) with a linear range of 0.1-8 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.

Bioactivity-FACS
 CD4 FACS

1e5 of Mouse Anti-CD4 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 647-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA2H8) and negative control protein respectively, AF647 signal was used to evaluate the binding activity (QC tested).

 CD4 FACS

1e5 of Mouse Anti-CD4 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 647-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA2H8) and negative control protein respectively, AF647 signal was used to evaluate the binding activity (QC tested).

  • Background
    CD4 is a glycoprotein that serves as an essential co-receptor on the surface of T lymphocytes (T cells), particularly helper T cells. It plays a critical role in the immune system by recognizing and binding to major histocompatibility complex (MHC) class II molecules on antigen-presenting cells (APCs), thereby facilitating T cell activation and immune response.
  • Clinical and Translational Updates

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  • Number of Drugs in Clinical Trials:23 Details
  • Latest Research Phase:Approved

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