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Your Position: Home > Antibody > CD16 > CD6-CH29G1

Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), premium grade

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  • Source
    Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), premium grade (CD6-CH29G1) is recombinantly produced from CHO cells.
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Isotype
    Mouse IgG1 | Mouse kappa
  • Conjugate

    Unconjugated

  • Specificity
    This product is a specific antibody that specifically reacts with CD16/Fc gamma RIII.
  • Endotoxin
    Less than 0.002 EU per μg by the LAL method / rFC method.
  • Protein A
    <5 ppm of protein tested by ELISA.
  • Host Cell Protein
    <0.5 ng/μg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Purity

    >95% as determined by SDS-PAGE.

  • Sterility
    Negative
  • Mycoplasma
    Negative.
  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    For long term storage, the product should be stored at liquid state at -70°C.

    This product is stable after storage at:

    1. -70°C for 24 months under sterile condition;
    2. 2-8℃ for 12 months under sterile condition.
SDS-PAGE
CD16 SDS-PAGE

Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-CELL BASE
 CD16 CELL

Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone 3G8), premium grade (Cat. No. CD6-CH29G1) stimulates Human CD16a (158V) (Luc) Jurkat Reporter Cell. The typically EC50 for this effect is 57.70 ng/mL (QC tested).

Bioactivity-ELISA
 CD16 ELISA

Immobilized Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), premium grade (Cat. No. CD6-CH29G1) with a linear range of 0.1-2 ng/mL (Routinely tested).

Bioactivity-FACS
 CD16 FACS

Flow cytometric analysis of Human peripheral blood mononuclear cell respectively staining with Monoclonal Anti-CD16 Antibody, Mouse IgG1 (Clone: 3G8), premium grade (Cat. No. CD6-CH29G1) at 10 μg/mL (1 μg of the antibody corresponds to labeling of 1e6 cells in a final volume of 100 µL) and PE anti-mouse IgG1 Antibody, compared with isotype control antibody. PE signal was used to evaluate the binding activity (Routinely tested).

  • Background
    CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
  • Clinical and Translational Updates

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