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The kit is developed for quantitative detection of natural and recombinant mouse IFN-gamma in serum, plasma and cell culture supernatants.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | Mouse IFN-gamma |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Mouse |
| Sensitivity | 3 pg/mL |
| Assay Time | 2.75 hr |
| Sample volume | 50 μL |
| Range | 9.4 pg/mL-600 pg/mL |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of mouse IFN-gamma is less than 3 pg/mL.
Ten replicates of each of three samples containing different mouse IFN-gamma concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Three samples containing different concentrations of mouse IFN-gamma were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant mouse IFN-gamma was spiked into three mouse serum samples, and then analyzed. The average recovery of IFN-gamma for serum samples is 100.2%.
| ID | Components | Size |
| CEA251-C01 | Pre-coated Anti-mouse IFN-gamma Antibody Microplate | 1 plate (8×12 strips) |
| CEA251-C02 | Mouse IFN-gamma Standard | 6 ng×2 |
| CEA251-C03 | Biotin-Anti-mouse IFN-gamma Antibody Con. Solution | 100μL |
| CEA251-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA251-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA251-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA251-C07 | 20×Washing Buffer | 50 mL |
| CEA251-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA251-C09 | Substrate Solution | 12 mL |
| CEA251-C10 | Stop Solution | 6 mL |
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