로그인 회원가입
icon_bulk_order대량 주문 Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Protein > Alpha-Synuclein > ALN-M51H3

Mouse Alpha-Synuclein Pre-formed Fibrils Protein, His Tag

Order Now

  • Synonym
    SNCA, NACP, PARK1, alpha-Synuclein
  • Source
    Mouse Alpha-Synuclein Pre-formed Fibrils Protein, His Tag(ALN-M51H3) is expressed from E. coli cells. It contains AA Met 1 - Ala 140 (Accession # O55042-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Alpha-Synuclein Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.4 kDa. The protein migrates as 19-21 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Application
    1. Sonication Conditions, Dissolution, Aliquoting, Storage, and Notes for PFF
    Thawing: Thaw PFFs rapidly in a 37 °C water bath, or allow to thaw at room temperature.
    Aliquoting: Since PFFs are supplied as a suspension, pipette up and down thoroughly before aliquoting to ensure homogeneity.
    Storage: Store at -80 °C at all times; avoid storage at 4 °C or -20 °C, which can induce fibril depolymerization. * α-Syn fibrils cold-denatured to monomers at 0-20 °C and heat-denatured at 60-110 °C.
    Sonication: The sonication protocols below are based on cell-based assay conditions.
    -Option 1 (Probe Sonicator): Use a probe sonicator (SCIENTZ) at 10% power (-95 W), applying 60 pulses of 0.5 s on/0.5 s off (recommended).
    -Option 2 (Ultrasonic Bath): Sonicate in an ultrasonic cleaner (40 kHz, 200-400 W) at 37 °C for 1 hour; avoid performing the treatment at 4 °C or 20 °C.
    Note: The above sonication protocols are based on cell-based assays; other applications (e.g., in vivo injections) may require optimization-users should determine their optimal settings for their specific use case.
    2. Recommendations for PFF Use in Animal Models
    Minimize Freeze-Thaw Cycles: For animal studies, it is recommend to use the PFF at once or aliquot before use to avoid repeated freeze-thaw cycles.
    PFF Maintenance During Injections: During stereotaxic injections, keep the sonicated PFFs in a 37 °C water bath to prevent re-aggregation or sedimentation.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product should be stored at -70°C or room temperature for short storage. Do not store fibrils on ice or at 4°C;
    2. The unsonicated fibril is validated to be stable after storage at -70°C for 1 year under sterile conditions;
    3. The sonicated fibril should be stored at -70°C for not more than 8 weeks.
SDS-PAGE
Alpha-Synuclein SDS-PAGE

Alpha-Synuclein monomer on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Electron Microscope
 Alpha-Synuclein ELECTRON MICROSCOPE

Transmission electron microscopy (TEM) of Mouse Alpha-Synuclein Pre-formed Fibrils Protein, His Tag (Cat. No. ALN-M51H3). Fibril structure is visible on negative stain TEM images of ALN-M51H3.​

  • Background
    Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD). Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration.
  • Clinical and Translational Updates

Comments (0)

Drug Candidate Licensing

more

Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:20 Details
  • Latest Research Phase:Phase 3 Clinical

Datasheet & Documentation

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop