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The kit is developed for quantitative detection of natural and recombinant human Fas Ligand/TNFSF6/CD178 in serum, plasma and cell culture supernatants.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | Fas Ligand/TNFSF6 |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Human |
| Sensitivity | 56 pg/ml |
| Assay Time | 1.75 hr |
| Sample volume | 50 μL |
| Range | 125 pg/mL-8000 pg/mL |
| Sample Type | For the quantitative determination of human Fas Ligand in Cell Culture Supernatants, Plasma, Serum. |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Fas Ligand is less than 56 pg/mL.
Ten replicates of each of three samples containing different Fas Ligand concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Three samples containing different concentrations of Fas Ligand were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant Fas Ligand was spiked into three human serum samples, and then analyzed. The average recovery of Fas Ligand for serum samples is 97.23%.
| ID | Components | Size |
| CEA085-C01 | Pre-coated Anti-Fas Ligand Antibody Microplate | 1 plate |
| CEA085-C02 | Human Fas Ligand Standard | 8 μg×2 |
| CEA085-C03 | Biotin-Anti- Fas Ligand Antibody Con. Solution | 100 μL |
| CEA085-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA085-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA085-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA085-C07 | 20×Washing Buffer | 50 mL |
| CEA085-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA085-C09 | Substrate Solution | 12 mL |
| CEA085-C10 | Stop Solution | 6 mL |
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