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Your Position: Home > Kits > PSMA > CEA-B242

Human PSMA / FOLH1 ELISA Kit

For research use only.
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    Product Overview
    Human PSMA/FOLH1 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human PSMA that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
    1. Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
    2. High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
    3. Simplified and straightforward protocols and ready-to-use reagents to save assay time.
  • Application

    The kit is developed for quantitative detection of natural and recombinant human PSMA in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow
     PSMA Workflow
  • Product Specifications
    Assay TypeSandwich-ELISA
    AnalytePSMA/FOLH1
    Format96-wells plate breakable into 12 x 8 wells strips
    ReactivityHuman
    Sensitivity2 ng/ml
    Assay Time2.75 hr
    Sample volume50 μL
    Range3.125 ng/mL-200 ng/mL
    Sample TypeFor the quantitative determination of human PSMA in serum, plasma and cell supernatant.

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
 PSMA TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of PSMA is less than 2 ng/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of three samples containing different PSMA concentrations were tested in one assay. Acceptable criteria: CV<10%.

 PSMA INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Three samples containing different concentrations of PSMA were tested in independent assays. Acceptable criteria: CV<15%.

 PSMA INTER-ASSAY STATISTICS
Recovery

Recombinant PSMA was spiked into three human serum samples, and then analyzed. The average recovery of PSMA for serum samples is 100.5%.

 PSMA RECOVERY
Materials Provided
IDComponentsSize
CEA242-C01Pre-coated Anti-PSMA Antibody Microplate1 plate
CEA242-C02Human PSMA Standard40 μg×2
CEA242-C03Biotin-Anti-PSMA Antibody Con. Solution100 μL
CEA242-C04Biotin-Antibody Dilution Buffer8 mL
CEA242-C05Streptavidin-HRP Con. Solution500 μL
CEA242-C06Streptavidin-HRP Dilution Buffer15 mL
CEA242-C0720×Washing Buffer50 mL
CEA242-C08Sample Dilution Buffer15 mL×2
CEA242-C09Substrate Solution12 mL
CEA242-C10Stop Solution6 mL
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:5 Details
  • Number of Drugs in Clinical Trials:69 Details
  • Latest Research Phase:Approved

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