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| Assay Type | Inhibition-TR-FRET |
| Analyte | Anti-IL-17A Neutralizing Antibody |
| Format | 100T/500T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | IC50=6.1nM |
| Standard Curve Range | 0.13 nM-133.33 nM |
| Assay Time | 1 hr |
| Suitable Sample Type | For Human IL-17A / IL-17RA Inhibitor Screening |
| Sample volume | 10 μL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| FRTP011-C01 | Human IL-17A Protein Europium-chelate | 100 tests/500 tests |
| FRTP011-C02 | FA Labeled Human IL-17RA Protein | 100 tests/500 tests |
| FRTP011-C03 | Anti-IL-17A Neutralizing Antibody | 10 μg/100 tests 50 μg/500 tests |
| DB-04 | TR-FRET Sample Dilution Buffer, pH7.4 | 50 mL/100 tests & 500 tests |
| DB-05 | TR-FRET Detection Buffer, pH7.4 | 50 mL/100 tests & 500 tests |
Inhibition of Europium-chelate labeled Human IL-17A: FA labeled Human IL-17RA binding by Anti-IL-17A Neutralizing Antibody
Premix serial dilutions of Anti-IL-17A Neutralizing Antibody (1:2 serial dilution, from 20 μg/mL to 0. 02 μg/mL (133.33-0.13 nM)) and Human IL-17A Protein Europium-chelate and incubate at room temperature (20℃-25℃) for 0.5 hours. Then add FA Labeled Human IL-17RA Protein and incubate at room temperature (20℃-25℃) for 0.5 hours. Detection was performed with IC50 of 6.1 nM. The assay was performed according to the above-described Datasheet (QC tested).
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