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| Assay Type | Inhibition-TR-FRET |
| Analyte | Anti-TSLP Neutralizing Antibody |
| Format | 100T/500T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | IC50=0.2264 nM |
| Standard Curve Range | 0.0033 nM-53.33 nM |
| Assay Time | 1 hr |
| Suitable Sample Type | For Human TSLP / IL-7Rα & TSLP R Inhibitor Screening |
| Sample volume | 10 μL |
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| FRTP012-C01 | Human TSLP Protein Europium-chelate | 100 tests/500 tests |
| FRTP012-C02 | FA Labeled Human IL-7 R alpha & TSLP R | 100 tests/500 tests |
| FRTP012-C03 | Anti-TSLP Neutralizing Antibody | 25 μg/100 tests 125 μg/500 tests |
| DB-04 | TR-FRET Sample Dilution Buffer, pH7.4 | 50 mL/100 tests & 500 tests |
| DB-05 | TR-FRET Detection Buffer, pH7.4 | 50 mL/100 tests & 500 tests |
Inhibition of Europium-chelate labeled human TSLP: FA labeled human IL-7 R alpha & TSLP R binding by Anti-TSLP Neutralizing Antibody
Premix serial dilutions of Anti-TSLP Neutralizing Antibody (1:2 serial dilution, from 8 μg/mL to 0.0005 μg/mL (53.33-0.0033 nM)) and Human TSLP Protein Europium-chelate and incubate at room temperature (20℃-25℃) for 0.5 hours. Then add FA Labeled Human IL-7 R alpha & TSLP R and incubate at room temperature (20℃-25℃) for 0.5 hours. Detection was performed with IC50 of 0.2264nM. The assay was performed according to the above-described Datasheet (QC tested).
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