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Your Position: Home > Antibody > CD14 > CD4-AMY2061

APC-Labeled Monoclonal Anti-Human CD14 Antibody, Mouse IgG1 (20F9E3) (0.03% Proclin)

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  • Source
    Monoclonal Anti-Human CD14 Antibody, Mouse IgG1 (20F9E3) is a mouse monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.
  • Application
    Flow Cytometry (Evaluation of the expression of CD14 on Human cells).
  • Clone
    20F9E3
  • Species
    Mouse
  • Isotype
    Mouse IgG1 | Mouse kappa
  • Specificity
    This product is a specific antibody specifically reacts with CD14 protein.
  • Reactivity

    Human

  • Immunogen

    Purified Human CD14 Protein

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Isotype Control

    The Isotype control is sold separately and you can search for Cat. No. DNP-AFM1 for product information.

  • Recommended Dilution
    1:50
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.2% BSA, 0.03% Proclin300 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months after reconstitution.
    3. 2-8 °C for 12 months after reconstitution.
Bioactivity-FACS
 CD14 FACS

Flow cytometric analysis of Human peripheral blood monocytes staining with APC-Labeled Monoclonal Anti-Human CD14 Antibody, Mouse IgG1 (20F9E3) (Cat. No. CD4-AMY2061) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. APC signal was used to evaluate the binding activity (QC tested).​

  • Background
    Cluster of differentiation 14 (CD14), is a cell surface glycoprotein, and is a is a component of the innate immune system. CD14 is a myelomonocytic differentiation antigen preferentially expressed on monocytes, macrophages, and activated granulocytes. CD14 exists in two forms. Either it is anchored into the membrane by a glycosylphosphatidylinositol tail (mCD14) or it appears in a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48 kDa) or is directly secreted from intracellular vesicles (56 kDa). CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). CD14 has been proposed to be involved in various biological processes, including transportation of other lipids, cell-cell interaction during different immune responses, as well as recognition of apoptotic cells. Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. CD14+ cells are monocytes that can differentiate into a host of different cells. CD14 has been shown to interact with Lipopolysaccharide-binding protein.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Phase 2 Clinical

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